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September 07, 2023
Category Connection: reagentes bioquímicos
Brief: Discover the versatile BES Buffer Free Acid (10191-18-1), a high-purity biochemical buffer essential for molecular biology and diagnostic assays. Learn about its applications in DNA transfection, protein studies, and more.
Related Product Features:
  • O ácido livre BES Buffer (CAS 10191-18-1) é um tampão zwitteriônico com uma faixa de pH de 6,4-7,8, ideal para pesquisa bioquímica.
  • Used in calcium phosphate-mediated DNA transfection for stable cell transformation.
  • Suitable for diagnostic assay manufacturing and protein studies due to its minimal interference.
  • Acts as a cross-linking agent in sulfonated polyimide membranes.
  • White crystalline powder with high solubility in water (1M at 20°C).
  • Melting point of 150-155°C and pKa of 7.1 at 25°C.
  • Low absorbance at 260 nm (≤0.05), making it suitable for UV-sensitive applications.
  • Compatible with Good's buffers, ensuring wide applicability in biological studies.
Perguntas frequentes:
  • What is the pH range of BES Buffer Free Acid?
    BES Buffer Free Acid has a useful pH range of 6.4 to 7.8, making it ideal for a variety of biochemical applications.
  • How is BES Buffer used in DNA transfection?
    BES Buffer is used in calcium phosphate-mediated DNA transfection, where it facilitates the slow formation of calcium phosphate-DNA complexes, enhancing stable cell transformation efficiency.
  • Is BES Buffer suitable for protein studies?
    Yes, BES Buffer is suitable for protein studies, though it may reduce color development in BCA protein assays by 30-35% at 50 mM due to potential Cu2+ binding.
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